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gfp 2a cre aavs  (Vector Biolabs)


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    Vector Biolabs gfp 2a cre aavs
    Gfp 2a Cre Aavs, supplied by Vector Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gfp 2a cre aavs/product/Vector Biolabs
    Average 95 stars, based on 4 article reviews
    gfp 2a cre aavs - by Bioz Stars, 2026-02
    95/100 stars

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    ( A ) Schematic of the protocol for experiments in B – K . ( B ) Sagittal schematic diagrams showing retro-AAV2-CRE-GFP injection into the bilateral VLO and <t>AAV-DIO-hM3Dq-mCherry</t> (or AAV-DIO-mCherry) injection into the bilateral Sm in mice. Photomicrograph of coronal section showing Cre-dependent mCherry + signals in the bilateral Sm (low magnification) and both <t>retrograde</t> labeled and Cre-dependent mCherry double-labeled neurons in the Sm (higher magnification). Scale bar: 200 μm for low magnification, 50 μm for high-magnification. ( C – F ) Activation of the Sm-VLO projection pathway by chemogenetic manipulation produced an antianxiodepressive effect in EPM ( C ), FST ( E ), and TST ( F ), but not in OFT ( D ). *P < 0.05, ***P < 0.001, 2-sided Student’s t test; n = 6 (mCherry) and 13 (hM3Dq). ( G ) Sagittal schematic diagrams and photomicrograph of coronal section showing AAV1-Cre injection into the bilateral Sm and AAV-DIO-hM3Dq-mCherry (or AAV-DIO-mCherry) injection of the bilateral VLO in mice. Scale bar: 500 μm for low magnification, 100 μm for high magnification. ( H – K ) Chemogenetic activation of the VLO neurons receiving projection from Sm produced an antianxiodepressive effect in EPM ( H ), FST ( J ), and TST ( K ), but not in OFT ( I ). *P < 0.05, **P < 0.01, 2-sided Student’s t test; n = 6 (mCherry) and 12 (hM3Dq).
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    ( A ) Schematic of the protocol for experiments in B – K . ( B ) Sagittal schematic diagrams showing retro-AAV2-CRE-GFP injection into the bilateral VLO and AAV-DIO-hM3Dq-mCherry (or AAV-DIO-mCherry) injection into the bilateral Sm in mice. Photomicrograph of coronal section showing Cre-dependent mCherry + signals in the bilateral Sm (low magnification) and both retrograde labeled and Cre-dependent mCherry double-labeled neurons in the Sm (higher magnification). Scale bar: 200 μm for low magnification, 50 μm for high-magnification. ( C – F ) Activation of the Sm-VLO projection pathway by chemogenetic manipulation produced an antianxiodepressive effect in EPM ( C ), FST ( E ), and TST ( F ), but not in OFT ( D ). *P < 0.05, ***P < 0.001, 2-sided Student’s t test; n = 6 (mCherry) and 13 (hM3Dq). ( G ) Sagittal schematic diagrams and photomicrograph of coronal section showing AAV1-Cre injection into the bilateral Sm and AAV-DIO-hM3Dq-mCherry (or AAV-DIO-mCherry) injection of the bilateral VLO in mice. Scale bar: 500 μm for low magnification, 100 μm for high magnification. ( H – K ) Chemogenetic activation of the VLO neurons receiving projection from Sm produced an antianxiodepressive effect in EPM ( H ), FST ( J ), and TST ( K ), but not in OFT ( I ). *P < 0.05, **P < 0.01, 2-sided Student’s t test; n = 6 (mCherry) and 12 (hM3Dq).

    Journal: JCI Insight

    Article Title: Activation of ventrolateral orbital cortex improves mouse neuropathic pain–induced anxiodepression

    doi: 10.1172/jci.insight.133625

    Figure Lengend Snippet: ( A ) Schematic of the protocol for experiments in B – K . ( B ) Sagittal schematic diagrams showing retro-AAV2-CRE-GFP injection into the bilateral VLO and AAV-DIO-hM3Dq-mCherry (or AAV-DIO-mCherry) injection into the bilateral Sm in mice. Photomicrograph of coronal section showing Cre-dependent mCherry + signals in the bilateral Sm (low magnification) and both retrograde labeled and Cre-dependent mCherry double-labeled neurons in the Sm (higher magnification). Scale bar: 200 μm for low magnification, 50 μm for high-magnification. ( C – F ) Activation of the Sm-VLO projection pathway by chemogenetic manipulation produced an antianxiodepressive effect in EPM ( C ), FST ( E ), and TST ( F ), but not in OFT ( D ). *P < 0.05, ***P < 0.001, 2-sided Student’s t test; n = 6 (mCherry) and 13 (hM3Dq). ( G ) Sagittal schematic diagrams and photomicrograph of coronal section showing AAV1-Cre injection into the bilateral Sm and AAV-DIO-hM3Dq-mCherry (or AAV-DIO-mCherry) injection of the bilateral VLO in mice. Scale bar: 500 μm for low magnification, 100 μm for high magnification. ( H – K ) Chemogenetic activation of the VLO neurons receiving projection from Sm produced an antianxiodepressive effect in EPM ( H ), FST ( J ), and TST ( K ), but not in OFT ( I ). *P < 0.05, **P < 0.01, 2-sided Student’s t test; n = 6 (mCherry) and 12 (hM3Dq).

    Article Snippet: To activate the Sm-VLO projection pathway, a retrograde AAV (AAV-hSyn-GFP-2A-Cre, 5.96 × 10 13 vg/mL, 300 nL per side, OBiO Technology Co., Ltd. Shanghai) was preinjected into the bilateral VLO.

    Techniques: Injection, Labeling, Activation Assay, Produced